Part:BBa_K4202016:Design

PHY-300PLK is a Escherichia coli-Bacillus subtilis shuttle vector

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal EcoRI site found at 37
  Illegal XbaI site found at 6
  Illegal PstI site found at 15
  Illegal PstI site found at 3830
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal EcoRI site found at 37
  Illegal PstI site found at 15
  Illegal PstI site found at 3830
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal EcoRI site found at 37
  Illegal BglII site found at 11
  Illegal BamHI site found at 27
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal EcoRI site found at 37
  Illegal XbaI site found at 6
  Illegal PstI site found at 15
  Illegal PstI site found at 3830
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal EcoRI site found at 37
  Illegal XbaI site found at 6
  Illegal PstI site found at 15
  Illegal PstI site found at 3830
  Illegal AgeI site found at 4709
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal BsaI site found at 2587
  Illegal BsaI site found at 4004
  

Design Notes

This is a plasmid without promotor and terminator.

Source

We obtained this from the FENGHUISHENGWU.